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Protocol

Microprobe system 을 이용한 IHC
Step Station Reagent Cycle Time Tem.
1 1, 2 XYLENE                 2 CYCLE 2 MIN   80℃
2 3~6 100%,95%,80% ALCOHOL        2~4 CYCLE 2 WASH       RT
3   DISTILLED WATER   3 WASH    RT
4 (OPTION)  ANTIGEN RETRIEVAL          
  COOLING   20 MIN    RT
DISTILLED WATER   2 WASH     RT
5 9 PEROXIDASE BLOCKING   5 MIN    45℃
6 7,8 TBS TWEEN BUFFER              2 CYCLE 2 WASH     RT
7 9 PROTEIN BLOCKER   5 MIN    45℃
8 9 PRIMARY ANTIBODY   20 MIN-30MIN     45℃
9 7,8 TBS TWEEN BUFFER 2 CYCLE 2 WASH     RT
10 10 SECONDARY ANTIBODY   5 MIN    45℃
11 7,8 TBS TWEEN BUFFER 2 CYCLE 2 WASH     RT
12 10 STREP HRP DETECTION SYS   5 MIN   45℃
13 7,8 TBS TWEEN BUFFER 2 CYCLE 2 WASH     RT
14 10 CHROMOGEN (AEC)         RT
15 7,8 TBS TWEEN BUFFER 2 CYCLE 2 WASH     RT
16 12 AUTO HEMATOXYLIN   1 MIN    RT
17 7,8 TBS TWEEN BUFFER 2 CYCLE 2 WASH     RT
18   MOUNTING      

 

 

14 step 에서DAB 를 사용하실 경우에는 탈수 과정을 거치고, 비수용성MOUNTING 처리 

 

    관리자 DATE   2016-12-15 14:27:27